View Counter: Abstract | 32 | times, and

PRIMACY OF LIQUID MEDIUM TECHNIQUE ON PROTOCORM LIKE BODIES PROPAGATION OF PhalaenopsiS SP ORCHIDS IN TISSUE CULTURE

Budi Kriswanto, Sigit Soeparjono, Didik Pudji Restanto

Abstract


Tissue culture have been used for plant propagation generally, and the medium has been important role in its growth. Vegetative propagation on Phalaenopsis sp orchids can be through the protocorm like bodies (PLB). Medium of affect on propagation of PLB was carried out on medium type, kind of basal medium and concentrations ratio of naphtaleneacetic acid (NAA) and benzylamino purine (BAP). The experiment used Completely Randomized Factorial Design with 3 replications and continued with the Duncan Multiple Range Test (DMRT) if there were significant differences. The results showed that the best callus formed in a combination of solid medium type and Murashige & Skoog (MS) basal medium was 100%. The most number of PLB produced from a combination of liquid medium types and a concentration ratio of NAA 1 mgL-1 and BAP 5 mgL-1, the most number of plantlet produced from a combination of MS basal medium and the concentrations ratio of NAA 0.1 mgL-1 and BAP 0.1 mgL-1, the number of PLB germination and PLB with leaves were influenced by each single factor.


Keywords


liquid medium; Phalaenopsis; Naphtaleneacetic Acid; Benzylamino Purine; Protocorm Like Bodies (PLB); Regeneration

Full Text:

PDF

References


Rahayu E.M.D, 2015. Konservasi anggrek bulan ( Phalaenopsis spp .) di Pusat Konservasi Tumbuhan Kebun Raya-LIPI, Bogor, 1, 1847–1850. https://doi.org/10.13057/psnmbi/m010816

Young P.S., H.N. Murthy, P. K. Yoeup, 2000. Mass Multiplication Of Protocorm-Like Bodies Using Bioreactor System And Subsequent Plant Regeneration In Phalaenopsis. Plant Cell, Tissue and Organ Culture, 63(1), 67–72. https://doi.org/10.1023/A:1006420116883

Hvoslef-Eide A., W. Preil, 2005. Liquid Culture Systems For In Vitro Plant Propagation. Springer, 588. https://doi.org/10.1017/CBO9781107415324.004

Suthar R. K., N. Habibi, S.D. Purohit, 2011. Influence Of Agar Concentration And Liquid Medium On In Vitro Propagation of Boswellia serrata Roxb. Indian Journal of Biotechnology, 10(2), 224–227.

Rupawan I.M., Z. Basri, M. Bustami, 2014. The Growth of Vanda Orchid ( Vanda sp ) on Various Medium Composition Via In Vitro Culture, 2(5), 488–494.

Silva P.A.K.X.D.M., S. Callegari-Jacques, M. H. Bodanese-Zanettini, 2000. Induction and identification of polyploids in Cattleya intermedium Lindl. (orchidaceae) by in vitro techniques. Ciência

Rural, 30(1), 105–111. https://doi.org/10.1590/S0103-84782000000100017

Niknejad A., M.A. Kadir, S.B. Kadzimin, 2009. In Vitro Plant Regeneration From Protocorms-Like Bodies ( Plbs ) And Callus of Phalaenopsis gigantea ( Epidendroideae : Orchidaceae ). The 6th National Biotechnology Congress of Iran, 10(56), 0–4. https://doi.org/10.5897/AJB10.2597

Gunawan L.W., 1988. Teknik Kultur Jaringan Tumbuhan. Institut Pertanian Bogor.

D. Meilasari, Iriawati, 2016. Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian.’ Journal of Mathematical and Fundamental Sciences, 48(3), 204–212.https://doi.org/10.5614/j.math.fund.sci.2016.48.3.2

Yeung E.C., T. A. Thorpe, C. J. Jensen. In Vitro Fertilization and Embryo Culture. in Plant Tissue Culture. Methods and Applications in Agriculture. Academic Press Inc. New York.1981.

George E.F., D.J.M Puttock, H.J. George, 1987. Plant Culture Medium. Volume 1. Formulations and Uses. Exegetic Limited, Edington, Westbury, England.

Arditti J., A. D. Krikorian, 1996. Orchid Micropropagation: The Path From Laboratory To Commercialization And An Account Of Several Unappreciated Investigators. Botanical Journal of the Linnean Society, 122(3), 183–241. https://doi.org/10.1006/bojl.1996.0059

Salisbury F.B.. and C.W., Ross. 1991. Plant Physiology. Fourth edition. Wadsworth Publishing Company. Belmont. California. A Division of Wadsworth Inc.

Bidwell R.G.S., 1979. Plant Physiology. MacMillan Publishing Co., Inc. New York., Collier Macmillan Publishers, London.

Danso K., K. Ayeh, V. Oduro, S. Amiteye, H. Amoatey, 2008. Effect of 6-Benzylaminopurine and -Naphthalene Acetic Acid on In vitro Production of MD2 Pineapple Planting Materials. World Applied Sciences Journal, 3(4), 614–619.

Palama T. L., P. Menard, I. Fock, Y.H. Choi, E. Bourdon, J. Govinden-Soulange, H. Kodja, 2010. Shoot Differentiation From Protocorm Callus Cultures Of Vanilla Planifolia (Orchidaceae): Proteomic And Metabolic Responses At Early Stage. BMC Plant Biology, 10, 82.

Su Y.H., Y.B. Liu, X.S. Zhang, 2011. Auxin-cytokinin interaction regulates meristem development. Molecular Plant, 4(4), 616–625. https://doi.org/10.1093/mp/ssr007

Ajijah N., 2016. Pengaruh Komposisi Medium Dasar Dan Jenis Eksplan Terhadap Pembentukan Embrio Somatik Kakao, J TIDP 3(3), 127–134.

Yih Ng C, N. M. Saleh, F. Q. Zaman, 2010. In Vitro Multiplication Of The Rare And Endangered Slipper Orchid , Paphiopedilum rothschildianum ( Orchidaceae ), 9(14), 2062–2068. https://doi.org/10.5897/AJB09.1535https://doi.org/10.1186/1471-2229-10-82

Park S., H.N. Murthy, K. Paek, 2002. Rapid Propagation Of Phalaenopsis From Floral Stalk-Derived Leaves, (April), 168–172. https://doi.org/10.1079/IVP2001274

Kauth P.J., D. Dutra, T.R. Johnson, S.L. Stewart, M.E. Kane, W.A. Vendrame, 2014. Techniques and applications of in vitro orchid seed germination. Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues., 375–391. October, Retrieved from http://www.researchgate.net/profile/Wagner_Vendrame/publication/257385365_Techniques_and_applications_of_in_vitro_orchid_seed_germination/links/544a9d4e0cf2bcc9b1d2f7c5.pdf

Tomar R.S., 2018. Role of Vitamins in Plant Growth and their Impact on Regeneration of Plants under Invitro Condition. International Journal for Research in Applied Science and Engineering Technology, 6(3), 423–426. https://doi.org/10.22214/ijraset.2018.3068

Liu G., E.K. Gilding, I.D. Godwin, 2013. Additive Effects of Three Auxin and Copper On Sorghum In Vitro Root Induction. In Vitro Cell. Dev.Biol. Plant 49:191-197..

Yu J., W. Liu, J. Liu, P. Qin, L. Xu, 2017. Auxin Control of Root Organogenesis from Callus in Tissue Culture. Frontiers in Plant Science, 8 (August), 1–4.. https://doi.org/10.3389/fpls.2017.01385




DOI: https://doi.org/10.24233/BIOV.6.1.2020.169

Refbacks

  • There are currently no refbacks.


E-ISSN: 2477-1392

Lisensi Creative Commons

BIOVALENTIA: Biological Research Journal © 2015-2019 Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University is licensed under a License Creative Commons Attribution-ShareAlike 4.0 International