DNA Extraction of Sumatran Striped Rabbit from Tissue Samples

Rio Firman Saputra, Indra Yustian, Arum Setiawan, Enggar Patriono

Abstract


The Sumatran Striped Rabbit (Nesolagus netscheri ) is  a naturally rare, which is a protected animal based on Government Regulation Number 7 of 1999 and renewal of Permen LHK No. P.106 which are categorized as Data Deficient  by the IUCN (International Union of Conservation of Nature) since 2019. Samples were obtained from Pagar Alam, South Sumatra. Most commonly found at elevations  above 600 m in montane and sub-montane primary habitat. Molecular genetic characterization of sumatran striped rabbit is to know genetic information and genetic identification of N. netscheri. Primers 12S rRNA were used in this study to characterize Sumatran striped rabbit. A 1 band were detected ranged from 900 to 1000 bp. Molecular markers represent reliable tools which may have a great impact in rabbit breeding and genetic improvement of rabbits. Molecular markers on Nesolagus netscheri is expected to tools in the identification of Sumatran striped rabbits from South Sumatra.

Keywords


Nesolagus netscheri; South Sumatra; Sumatran Striped Rabbit; PCR; 12S rRNA

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References


Dinets, V. 2010. Observation of Sumatran Striped Rabbit (Nesolagus netscheri) in the Wild. Mammalia. 74, 1.

El-Sabrout K., El-Seedy A., Shebl M. K., Soliman F. N. K., Azza El-Sebai, 2014 Molecular markers and productive performance relations for line V and Alexandria rabbits under Egyptian environmental conditions. Int J Life Sci Biotechnol Pharma Res 3(1):345-361.

Flux, J. E. C. 1990. The Sumatran striped rabbit. in Rabbits, Hares and Pikas: Status Survey and Conservation Action Plan (eds J.A. Chapman & J.E.C. Flux), Halaman. 137–139. IUCN, Gland, Switzerland.

Ge, D., Yao, L., Xia, L., Zhang, L., dan Yang, Q. 2015. Geometric morphometric analysis of skull morphology reveals loss of phylogenetic signal at the generic level in extant lagomorphs (Mammalia: Lagomorpha). Contributions to Zoology, 84 (4) 267-284.

Gillespie, J. J., Johnston, J. S., Cannone§, J. J., dan Gutell§, R. R. 2006. Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of (Insecta: Hymenoptera): structure, organization, and retrotransposable elements Apis mellifera. Insect Molecular Biology (2006) 15 (5), 657–686.

Hoffmann, R.S. dan Smith, T.A. 2005. Lagomorpha: Leporidae. In: (D.E. Wilson and D.M. Reeder, eds.). Wilson, D.E and Reeder, D. M. (editors) (2005). Mammal Species of the World-A Taxonomic and Geographic Reference (Third ed.). Johns Hopkins University, Baltimore. Halaman. 185-211.

McCarthy, J. L., Fuller, T. K., McCarthy, K. P., Wibisono, H. T., dan Livolsi, M. C. 2012. Using camera trap photos and direct sightings to identify possible refugia for the Vulnerable Sumatran striped rabbit Nesolagus netscheri. Oryx 46(3): 438–441.

McCarthy, J., Holden, J., Martyr, D. & McCarthy, K. 2019. Nesolagus netscheri. The IUCN Red List of Threatened Species 2019: e.T14662A45178557. http://dx.doi.org/10.2305/IUCN.UK.2019-2.RLTS.T14662A45178557.en. Downloaded on 2 September 2019.

Meijaard, E. dan Sugardjito, J. 2008. Nesolagus netscheri. Di IUCN Red List of Threatened. Http://www.iucnredlist.org [diakses tanggal 12 September 2017].

Melton, T., dan Holland, C. 2007. Routine Forensic Use of the Mitochondrial 12S Ribosomal RNA Gene for Species Identification. J Forensic Sci, 52 (6).

Olson, L. E., Sargis, E. J., dan Martin, R. D. 2005. Intraordinal phylogenetics of treeshrews (Mammalia: Scandentia) based on evidence from the mitochondrial 12S rRNA gene. Molecular Phylogenetics and Evolution, 35 (2005) 656–673.

Page, R. D. M., Cruickshank R., dan Johnson†, K. P. 2002. Louse (Insecta: Phthiraptera) mitochondrial 12S rRNA secondary structure is highly variable. Insect Molecular Biology (2002) 11(4), 361–369.

Rastogi, G., Dharne, M., Bharde, A., Pandav, V. S., Ghumatkar, S. V., Krishnamurthy, R., Patole, M. S., dan Shouche, S.Y. 2004. Species determination and authentication of meat samples by mitochondrial 12S rRNA gene sequence analysis and conformation sensitive gel electrophoresis. Current Science, 87 (9).

Setiawan, A., Iqbal, M., Komarudin, Saputra, R. F., Setiawan, D., dan Yustian, I. 2018. New reports of the presence and ecology of the Sumatran Striped Rabbit (Nesolagus netscheri) in South Sumatra. Mammalia, 82(6): 589-591. Retrieved 16 Nov. 2018, doi:10.1515/mammalia-2017-0064.

Surridge, A. K., Timmins, R. J., Hewitt, G. M. dan Bell, D. J. 1999. Striped rabbits in Southeast Asia. Nature 400: 726.

Tougard, C., Delfosse, T., Hanni, C., dan Montgelard, C .2001. Phylogenetic Relationships of the Five Extant Rhinoceros Species (Rhinocerotidae, Perissodactyla) Based on Mitochondrial Cytochrome b and 12S rRNA Genes. Molecular Phylogenetics and Evolution. Vol. 19, No. 1. Halaman 34–44.

Wang, H. Y., Tsai, M. P., Tu, M. C., dan Lee, S. C. 2000. Universal Primers for Amplification of the Complete Mitochondrial 12S rRNA Gene in Vertebrates. Zoological Studies, 39(1).Halaman 61-66.

Wilson, D. E., dan Reeder, D. M. 2005. Mammal species of the world : a taxonomic and geographic reference. The Johns Hopkins University Press. United States of America.

Yang, L., Tan,Z., Wang, D., Xue, L., Guan, M., Huang, T., dan Li, R. 2014. Species identification through mitochondrial rRNA genetic analysis. Scientific Reports, 4 : 4089.




DOI: https://doi.org/10.24233/BIOV.5.2.2019.148

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